Search results for "methods [Biological Assay]"
showing 10 items of 243 documents
-Infinity robust controller design for the synchronization of master-slave chaotic systems with disturbance input
2012
Published version of an article from the journal: Modeling, Identification and Control. Also available from the publisher: http://dx.doi.org/10.4173/mic.2012.1.3 This paper is concerned with the robust control problems for the synchronization of master-slave chaotic systems with disturbance input. By constructing a series of Lyapunov functions, novel H1 robust syn- chronization controllers are designed, whose control regulation possess the characteristic of simpleness and explicitness. Finally, numerical simulations are provided to demonstrate the e ectiveness of the proposed techniques.
An LMI approach to vibration control of base-isolated building structures with delayed measurements
2010
In this article, we address a convex optimisation approach to the problem of state-feedback H∞ control design for vibration reduction of base-isolated building structures with delayed measurements, where the delays are time-varying and bounded. An appropriate Lyapunov-Krasovskii functional and some free-weighting matrices are utilised to establish some delay-range-dependent sufficient conditions for the design of desired controllers in terms of linear matrix inequalities. The controller, which guarantees asymptotic stability and an H∞ performance, simultaneously, for the closed-loop system of the structure, is then developed. The performance of the controller is evaluated by means of simula…
Impact of intravascular enhancement, heart rate, and calcium score on diagnostic accuracy in multislice computed tomography coronary angiography.
2005
PURPOSE: To assess the effect of intravascular enhancement, heart rate, and calcium score on diagnostic accuracy in the detection of significant coronary artery stenosis using 16-row multislice computed tomography (MSCT). MATERIALS AND METHODS: One hundred patients (88 males; 59+/-11 years) with suspected coronary artery disease who had undergone conventional coronary angiography (CA) and MSCT-CA were retrospectively enrolled for the study. Patients underwent a MSCT-CA, with the following protocol: collimation 16x0.75 mm, gantry rotation time 420 ms, feed/rotation 2.8 mm, kV 120, mAs 400-500. The protocol for contrast material administration was 100 ml of Iodixanol 320 mgI/ml at 4 ml/s and …
RNAPol-ChIP: a novel application of chromatin immunoprecipitation to the analysis of real-time gene transcription.
2004
We describe a procedure, RNAPol-ChIP, to measure actual transcriptional rate. It consists of the detection, by chromatin immunoprecipitation (ChIP), of RNA polymerase II within the coding region of genes. To do this, the DNA immunoprecipitated with polymerase antibodies is analysed by PCR, using an amplicon well within the coding region of the desired genes to avoid interferences with polymerase paused at the promoter. To validate RNAPol-ChIP, we compare our results to those obtained by classical methods in several genes induced during either liver regeneration or acute pancreatitis. When short half-life mRNA genes are studied (e.g. c-fos and egr1), RNAPol-ChIP gives results similar to thos…
Construct validity of the anxiety sensitivity index-3 in clinical samples
2012
"Using two clinical samples of patients, the presented studies examined the construct validity of the recently revised Anxiety Sensitivity Index-3 (ASI-3). Confirmatory factor analyses established a clear three-factor structure that corresponds to the postulated subdivision of the construct into correlated somatic, social, and cognitive components. Participants with different primary clinical diagnoses differed from each other on the ASI-3 subscales in theoretically meaningful ways. Specifically, the ASI-3 successfully discriminated patients with anxiety disorders from patients with nonanxiety disorders. Moreover, patients with panic disorder or agoraphobia manifested more somatic concerns …
Componimenti misti di storia dell’arte e d’invenzione: gli antichi maestri nella pittura e nella novellistica italiana dell’Ottocento
2008
This article investigates the popular imagery of art-historical matters in Italian Romantic art and literature.
Patented and commercialized applications
2021
Abstract The commercialization of high added-value compounds recovery from food wastes deals with several issues such as laboratory research, scale-up problems, protection of intellectual properties, and development of market-destined applications. These issues are described in this chapter wherein a collection of commercially available compounds recovered from food by-products is presented. Verification of market existing products matching with patented processes was conducted using patent applicant name in each case. However, this matching as well as production characteristics may not be correct in all cases, as most companies typically are secretive about their methods of production and …
Aseptic Production vs. Terminal Sterilization
2000
The safety of substance use in intraocular surgery ideally requires the absence of all biologically-active components capable of causing an inflammatory reaction. Substance purity is achieved with isolation and cleansing methods (e.g., of the polysaccharide), which remove any biological component with infective potential. The methods of production for polysaccharide isolation should remove biological agents, regardless of its origin. Hyaluronic acid is isolated either from rooster combs or biological fermentation. Use of this substance showed the development of postoperative intraocular inflammatory reactions resulting from contaminations from both sources. OVD should have low endotoxin val…
FICC-Seq: a method for enzyme-specified profiling of methyl-5-uridine in cellular RNA.
2019
AbstractMethyl-5-uridine (m5U) is one the most abundant non-canonical bases present in cellular RNA, and in yeast is found at position U54 of tRNAs where modification is catalysed by the methyltransferase Trm2. Although the mammalian enzymes that catalyse m5U formation are yet to be identified via experimental evidence, based on sequence homology to Trm2, two candidates currently exist, TRMT2A and TRMT2B. Here we developed a genome-wide single-nucleotide resolution mapping method, Fluorouracil-Induced-Catalytic-Crosslinking-Sequencing (FICC-Seq), in order to identify the relevant enzymatic targets. We demonstrate that TRMT2A is responsible for the majority of m5U present in human RNA, and t…
Approaches to study yeast cell aging and death
2014
For millennia, yeast has been exploited to obtain fermentation products, such as foods and beverages. For c. 50 years, yeast has been an established model organism for basic and applied research, and more specifically, for c. 15 years, this unicellular organism has been applied to dissect molecular mechanisms of cell aging and programmed cell death. In this review, we present an overview of approaches to study cell aging and death in yeast, including lifespan assessments, calorie restriction, cell viability, survival, and death markers.